Journal: The Prostate

Article Title: The identification of a novel antibody for CD133 using human antibody phage display

doi: 10.1002/pros.23656

Figure Lengend Snippet: Case study of a patient with aggressive prostate cancer. A, CT scan of diffuse liver metastases that appeared in the presence of declining PSA after the patient failed treatment with Zytiga (abiraterone). B, Strong staining for CD133 was detected in the liver metastases using HA10. C, No staining for the AR was observed in the liver metastases using an antibody specific to the N-terminal domain of the AR. This antibody would detect both full-length AR and AR splice variants. D, Staining was observed for the neuroendocrine differentiation marker chromogranin A in the liver metastases. E, An original TURP specimen from the patient depicting poorly differentiated Gleason 4 + 5 cancer stained for CD133. Note the complete absence of CD133. F, Liver biopsy from the patient after failing treatment with carboplatin and taxotere documenting moderate staining for CD133. Scale bars, (B-D) 200 μm and (E-F) 60 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: The following primary antibodies and concentrations used were: CD133 HA10 antibody, rabbit monoclonal (1:100); androgen receptor SP107 (Sigma), rabbit monoclonal (1:200); chromogranin A PB9097 (Boster Biological Technology) rabbit polyclonal (1:1000); CD133/1 (AC133) (Miltenyi) mouse monoclonal (1:25).

Techniques: Computed Tomography, Staining, Marker

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 1 Serum adiponectin concentrations in rabbits. 1, Aorta non-injured rabbits on normal cholesterol diet; 2, aorta injured rabbits on high-cholesterol diet before adenovirus transfer; 3, after transfer Ad-bgal through intima; 4, after transfer Ad-APN through intima; 5, after transfer Ad-bgal through adventitia; 6, After transfer Ad-APN through adventitia. The symbol * indicates significant difference (P!0.01) vs serum adiponectin concentrations in aorta non-injured rabbits on normal cholesterol diet; † compared with rabbits before adenovirus transfer; and ‡ compared with rabbits after Ad-bgal transfer. The values are meansGS.E.M.

Article Snippet: For immunohistochemical analyses, paraffin-imbedded cross-sections (5 mm thick) were incubated with either rabbit adiponectin polyclonal antibody (Boster), mouse vascular adhesion molecule-1 (VCAM-1) monoclonal antibody (Santa Cruz), mouse intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (Santa Cruz), mouse collagen I (Abcam, Cambridge, MA, USA), or mouse collagen III (Calbiochem, Darmstadt, Germany) monoclonal antibody.

Techniques:

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 2 SDS-PAGE (A) and western blot analysis of the supernatant of cultured Pichia pastoris (B, using anti-His tag monoclonal antibody; C, using adiponectin polyclonal antibody). Line 1 is a blank pGAPZa Pichia vector (not containing adiponectin gene) sample control and lines 2–4 are samples from the supernatant of cultured Pichia pastoris containing the recombinant rabbit adiponectin gene. Line 5 contained the molecular weight markers (not visible in image of blot). Both anti-His antibody and adiponectin polyclonal antibody detected a single predominant protein at z42 kDa (containing 9.3 kDa a-factor signal peptide and 2.5 kDa C-terminal His-tag).

Article Snippet: For immunohistochemical analyses, paraffin-imbedded cross-sections (5 mm thick) were incubated with either rabbit adiponectin polyclonal antibody (Boster), mouse vascular adhesion molecule-1 (VCAM-1) monoclonal antibody (Santa Cruz), mouse intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (Santa Cruz), mouse collagen I (Abcam, Cambridge, MA, USA), or mouse collagen III (Calbiochem, Darmstadt, Germany) monoclonal antibody.

Techniques: SDS Page, Western Blot, Cell Culture, Plasmid Preparation, Control, Recombinant, Molecular Weight

Journal: Journal of Endocrinology

Article Title: Local adiponectin treatment reduces atherosclerotic plaque size in rabbits

doi: 10.1677/joe-06-0173

Figure Lengend Snippet: Figure 5 Effect of Ad-APN treatment on adhesion molecules in atherosclerotic rabbits 14 days after recombinant adenovirus transfer. A, Immunohistochemical detection of adiponectin, VCAM-1 and ICAM-1 in atherosclerotic lesions. Representative images are shown. 1, Ad-bgal transfer through intima. 2, Ad-APN transfer through intima. 3, Ad-bgal transfer through adventitia. 4, Ad-APN transfer through adventitia. The magnification is 400. B, mRNA expression of VCAM-1 and ICAM-1 in abdominal aortic tissue in Ad-bgal or Ad-APN-treated rabbits. The values are meansGS.E.M.

Article Snippet: For immunohistochemical analyses, paraffin-imbedded cross-sections (5 mm thick) were incubated with either rabbit adiponectin polyclonal antibody (Boster), mouse vascular adhesion molecule-1 (VCAM-1) monoclonal antibody (Santa Cruz), mouse intercellular adhesion molecule-1 (ICAM-1) monoclonal antibody (Santa Cruz), mouse collagen I (Abcam, Cambridge, MA, USA), or mouse collagen III (Calbiochem, Darmstadt, Germany) monoclonal antibody.

Techniques: Recombinant, Immunohistochemical staining, Expressing

Journal:

Article Title: Massive Destruction of Malaria-Parasitized Red Blood Cells despite Spleen Closure

doi: 10.1128/IAI.73.10.6390-6398.2005

Figure Lengend Snippet: (A to C) Trapping capacity of the spleen during P. chabaudi infection. Splenic uptake of pRBC (A), RBC (B), and BSA-TRITC (C) was analyzed by fluorescence microscopy on the days indicated (d0 to d35) after injection of 1 × 109 PKH-26-labeled pRBC or noninfected RBC or 500 μg BSA-TRITC. (D) Semiquantitative evaluation of fluorescence intensity for 10 cryosections per mouse with three to five mice per time after injection of PKH-26-labeled pRBC (red bars), noninfected RBC (green bars), and BSA-TRITC (blue bars). Each bar indicates the mean, and each error bar indicates 0.5 standard error of the mean. The horizontal lines indicate means ± standard errors of the means for background fluorescence for cryosections of mice that did not receive an injection. (E) Numbers of F4/80+ macrophages (black bars) and Gr-1+ granulocytes (red bars) expressed as percentages of the total spleen cells. Each bar indicates the mean, and each error bar indicates 0.5 standard error of the mean. One asterisk, P < 0.05 compared with day 0 p.i.; two asterisks, P < 0.01 compared with day 0 p.i.

Article Snippet: Specifically, cells were preincubated with anti-mouse CD16/CD32 (FcIII/II receptor) F c block (BD PharMingen, Heidelberg, Germany) for 3 min and then labeled with anti-mouse F4/80 (ImmunoKontact, Wiesbaden, Germany) or anti-mouse Gr-1 (BD PharMingen) fluorescein isothiocyanate-labeled monoclonal antibodies.

Techniques: Infection, Fluorescence, Microscopy, Injection, Labeling